biotinylation of membrane proteins

R. Woodward rw200 at cus.cam.ac.uk
Wed Apr 2 09:11:46 EST 1997


Dear All

I want to biotinylate some membrane proteins so that I can subsequently
perform some coimmunoprecipitation studies I have a method for 
biotinylation of proteins on  the cell surface but I would also like
to be able to label up proteins which are on the internal side
of the plasma membrane. My question is can I just homogenise my cells and
biotinylate my membrane prep or are there other things I have to do
, Any references?


Thanks in anticipation

Robert

rw200 at cus.cam.ac.uk

ee
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