Quantitation RT-PCR

ma ddsyr at NS.EAST.CN.NET
Wed Apr 2 06:11:14 EST 1997


Dear netter:
Could anyone tell me a good protocol to measure mRNA amount in plant tissues by RT-PCR?
I read a paper in Plant Mol Biol, they used a rat hepatoma cell total RNA as a control.
Is it possible to use the rRNA in plant tissue itself as a internal control and 
synthesized a specific primers to that?( which is like in Northern hybridization) Any 
suggestion or experiences for that is welcome.
Ma




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