Northern Hybridisation

VASANTHARAJAN JANAKIRAMAN vasant at cc.UManitoba.CA
Thu Apr 3 15:26:33 EST 1997


Hi everyone,

    I am looking into the expression of phenylpropanoid genes - PAL, COMT 
and CCOMT- specifically involved in the lignin synthesis, in the wheat 
cultivars resistant and susceptible to a leaf spot disease, using 
alfalfa phenylpropanoid cDNA clones- CCOMT, COMT and Rice -PAL cDNA 
clone as probes.
My question is - since the homologies between Alfalfa (Dicot) and wheat 
(Monocot) aren't that great, what are the conditions I would have to 
consider while performing Northern hybridisation?
I would like to perform the hyb. with total RNA isolated from the 
inoculated cultivars. 
Any suggestions/comments welcome.
my email add- vasant at ccu.umanitoba.ca
bye
vasanth



More information about the Methods mailing list