Green Fluorescent Protein chimera?

Nico Dantuma n.p.dantuma at biol.ruu.nl
Thu Apr 3 08:19:34 EST 1997


Sorry, the correct e-mail adress is n.p.dantuma at biol.ruu.nl

Nico Dantuma
>Hi,

>I was wondering to what extend it might be possible to mess up green
>fluorescent protein without losing  any fluorescence or at least
>having enough signal left to monitor. Is it for example possible to
>integrate a linker region in the protein? If so, what is the maximal
>length of this region and what is the position where it can be
>integrated? Is it possible to make a chimera with a different protein
>by extending the protein at either the amino or carboxyl terminus with
>the protein of interest? 

> Please let me know if you have any answers on the above mentioned
>questions or suggestions for alternate startiegies to use GFP as an
>epitope tag by generating protein chimera.

>Replies preferable by e-mail.

>Thanks,

>Nico Dantuma 






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