rat-1 question

[jstrassw at OPAL.TUFTS.EDU]

Hi Ole!
Well, if rat-1 are hard to transfect like primary human fibroblasts, the
only thing that worked for fibros is lipotaxi from Stratagene.  Very
expensife and you only get 1% tranfected anyhow....

good luck,
John


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John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
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On 3 Apr 1997, Ole Gjoerup wrote:

> Hello everyone,
> 
> I'm going to be transfecting rat-1 clone R12 cells for a reporter assay.
> I've heard that rat-1 cells can be extremely hard to transfect by CaPO4.
> DO you know of a better method ? or anyhow, what's your experience with
> rat-1 transfectability ?
> 
> Thanks for your help. Sincerely,
> 
> -Ole
> 
> ------------------------------------------------------------------------
> Ole Victor Foenss Gjoerup, Ph.D.       | Phone (+45)35322071
> Dept. of Molecular and Cellular        | FAX   (+45)33935220
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>