rat-1 question

jstrassw at OPAL.TUFTS.EDU jstrassw at OPAL.TUFTS.EDU
Thu Apr 3 11:03:14 EST 1997


Hi Ole!
Well, if rat-1 are hard to transfect like primary human fibroblasts, the
only thing that worked for fibros is lipotaxi from Stratagene.  Very
expensife and you only get 1% tranfected anyhow....

good luck,
John


------------------------------------------------------------------------
John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
Tufts - New England Medical Center     | Fax   (617) 636 6190
Box 166                                | Email:  jstrassw at opal.tufts.edu
Boston, MA 02111 USA                   |                              
     HTTP://WWW.healthsci.tufts.edu/microbiology/strass/JSpage.HTML
     *** Co-Author of "HyperBug" Microbiology Teaching Software ***
                                       |
Remember, Your employer is eavesdropping...  
------------------------------------------------------------------------

On 3 Apr 1997, Ole Gjoerup wrote:

> Hello everyone,
> 
> I'm going to be transfecting rat-1 clone R12 cells for a reporter assay.
> I've heard that rat-1 cells can be extremely hard to transfect by CaPO4.
> DO you know of a better method ? or anyhow, what's your experience with
> rat-1 transfectability ?
> 
> Thanks for your help. Sincerely,
> 
> -Ole
> 
> ------------------------------------------------------------------------
> Ole Victor Foenss Gjoerup, Ph.D.       | Phone (+45)35322071
> Dept. of Molecular and Cellular        | FAX   (+45)33935220
> Biology                                |
> Oester Farimagsgade 2A                 | Email: ovg at biobase.dk
> 1353 Koebenhavn K                      | http://biobase.dk/~ovg
> Denmark                                |
> ------------------------------------------------------------------------
> 
> 




More information about the Methods mailing list