SSCP

[Geoff Scopes]

In article <5hufj4$ajk at isnews.csc.calpoly.edu>, The Kindly One
<smccull at jurassic.fisher.calpoly.edu> writes
>Hello,
>
>Can someone point me to a reference(any form) for a good
>discussion of the idea/theory behind SSCP? A protocol(generic) 
>will be helpful too!!
>
>Thanks
>
>sdm
>
For a review of techniques (a few years old now though) see:
Prosser,J (1993) Detecting single base mutations. TIBTECH, 11:238-246.

More up to date, but without references is:
Forrest,S.,Cotton,R.,Landegren,U. and Southern,E. (1995) How to find all
those mutations. Nat. Genet. 10:375-376

Specifically on SSCP:
Sheffield, Val C. et al (1993) The sensitivity of Single-Strand
Conformation Polymorphism Analysis for the detection of single base
substitutions. Genomics, 16: 325-332


Regards,
--                                                       ______
Dr Geoff Scopes,
Research and Development Manager, Advanced Biotechnologies Limited,  
Units B1-B2, Longmead Business Centre, 
Blenheim Road, Epsom, Surrey KT19 9QQ, UK.
Tel:  01372 723 456             E.mail: geoff at adbio.demon.co.uk
Fax:  01372 741 414             WWW:    http://www.adbio.co.uk/