GFP and immunofluorescence

[Stephan Geley]

I am using GFP as cotransfection marker in transient transfection assays
which gives me a bright signal in live cells. However, when I try to fix
cells for immunostaining I always loose the GFP signal. So far I've used
Methnanol or Methanol/Acetone or Paraformaldehyd followed by .1% TX100
without any success. Can anyone out there in the cybercommunity give me
some advice? 
Thanks anyway,
Stephan