GFP and immunofluorescence

Stephan Geley s.geley at ICRF.ICNET.UK
Fri Apr 4 18:29:32 EST 1997


I am using GFP as cotransfection marker in transient transfection assays
which gives me a bright signal in live cells. However, when I try to fix
cells for immunostaining I always loose the GFP signal. So far I've used
Methnanol or Methanol/Acetone or Paraformaldehyd followed by .1% TX100
without any success. Can anyone out there in the cybercommunity give me
some advice? 
Thanks anyway,
Stephan



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