Best Hybridization Solutions for Southerns AND NORTHERNS?

Bradley Turner bsturner at MBCRR.HARVARD.EDU
Fri Apr 4 09:06:29 EST 1997


Hi Paul and other bionetters,

Does this advantage of using "Church" hybridization solutions extend
to Northern blots of RNA on positively charced nylon membranes
(HYbond-N+), i.e. for reducing background?

Does transfer of nucleic acids with the downward alkaline
method affect this recommendation?

Thank you,
Brad Turner

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		    Bradley Turner
	        Beth Israel Deaconess
                    Medical Center

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			bsturner at mbcrr.harvard.edu
330 Brookline Avenue		bturner at bidmc.harvard.edu
Boston, MA 02215, USA		turner at sprcore.bidmc.harvard.edu
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