Best Hybridization Solutions for Southerns AND NORTHERNS?

Bradley Turner bsturner at MBCRR.HARVARD.EDU
Fri Apr 4 09:06:29 EST 1997

Hi Paul and other bionetters,

Does this advantage of using "Church" hybridization solutions extend
to Northern blots of RNA on positively charced nylon membranes
(HYbond-N+), i.e. for reducing background?

Does transfer of nucleic acids with the downward alkaline
method affect this recommendation?

Thank you,
Brad Turner

		    Bradley Turner
	        Beth Israel Deaconess
                    Medical Center

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			bsturner at
330 Brookline Avenue		bturner at
Boston, MA 02215, USA		turner at

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