DNA isolation

Paul N Hengen pnh at ncifcrf.gov
Fri Apr 4 12:47:56 EST 1997


> I extract DNA from vegetable plant for RAPD analysis. This is done with
> standaard CTAB extraction. The disruption of the plant material is done
> by a powerdrill in a eppendorf tube. Does anybody outthere in cyberspace
> know a method to disrupt 96 plant samples at once or has some good
> ideas.
> 
> Greetings,
> 
> Stefan van Lier
> Technical assistent Molecular Biology.

Interesting! What size bit do you use?  How do you keep the drill
bit from making a hole in the bottom of the plastic eppendorf tube?

--
*******************************************************************************
* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
* - - -  Methods FAQ list -> ftp://ftp.ncifcrf.gov/pub/methods/FAQlist - - -  *
* -  TIBS column archive -> http://www-lmmb.ncifcrf.gov/~pnh/readme.html - -  *
* - The BEST Molecular Biology HomePage -> http://www-lmmb.ncifcrf.gov/~pnh/  *
*******************************************************************************



More information about the Methods mailing list