biotinylation of membrane proteins

Ian A. York iayork at panix.com
Sat Apr 5 16:55:08 EST 1997


In article <199704021533.JAA00983 at borcim.wustl.edu>,
brett <brett at BORCIM.WUSTL.EDU> wrote:
>
>Sure enough, you can biotinylate lysates too. BTW, I tried for months to
>specifically biotinylate *cell surface* proteins, but found that known
>cytoplasmic proteins, including actin, were biotinylated to detectable
>levels. After many dozens of experiments, I am convinced that this technique

Have you tried the same control with iodination, or some other surface
labelling technique?  In other words, is there any really clean method for
surfdace labelling?

Ian
-- 
      Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
      "-but as he was a York, I am rather inclined to suppose him a
       very respectable Man." -Jane Austen, The History of England



More information about the Methods mailing list