Best Hybridization Solutions for Southerns AND NORTHERNS

bsturner at MBCRR.HARVARD.EDU bsturner at MBCRR.HARVARD.EDU
Sat Apr 5 04:36:17 EST 1997


--Boundary (ID OE+bvk6sHw/CwyOPq14npw)
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Hi Paul and other bionetters,

Does this advantage of using "Church" hybridization solutions extend
to Northern blots of RNA on positively charced nylon membranes
(HYbond-N+), i.e. for reducing background?

Does transfer of nucleic acids with the downward alkaline
method affect this recommendation?

Thank you,
Brad Turner

**************************************************************
                    Bradley Turner
                Beth Israel Deaconess
                    Medical Center

Harvard Medical School          617-667-1215 phone
Division of Gastroenterology    617-667-2767 fax
Room Dana 536                   bsturner at mbcrr.harvard.edu
330 Brookline Avenue            bturner at bidmc.harvard.edu
Boston, MA 02215, USA           turner at sprcore.bidmc.harvard.edu
**************************************************************



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