Sequencing gel molecular wt. marker

kraev at bc.biol.ethz.ch kraev at bc.biol.ethz.ch
Sun Apr 6 08:49:05 EST 1997


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In article <dinakar-0104971740410001 at dong.tamu.edu>, dinakar at unix.tamu.edu
(Dinakar Bhattramaki) wrote:

> Hi,
>
> We need a molecular weight marker which could be run on a sequencing gel.
> Since we need to differentiate different bands which run close-by, the
> smallest range possible -like 2-4 base difference in the 100 to 200 bp
> region-is the ideal one. We use silver staining method (non-radioactive)
> to visualize the bands.
>

Try Research  Genetics "Sequamark" (expensive), otherwise find someone
working with
dinucleotide repeats and get a sequenced clone to be used as a marker.

I hope this helps,

--
Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
Phone 41-1-632-31-47
Fax 41-1-632-12-13
e-mail kraev at bc.biol.ethz.ch


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Date: Fri, 04 Apr 1997 14:44:05 +0100
From: kraev at bc.biol.ethz.ch (Alexander Kraev)
Subject: Re: Sequencing gel molecular wt. marker
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