Labelling -COOH groups with fluorosceinyl-Gly-amide: help!

graham at dbe.csiro.au graham at dbe.csiro.au
Sun Apr 6 04:26:06 EST 1997


--Boundary (ID V9XX7MM22hBGr57OMsi93Q)
Content-type: TEXT/PLAIN


<Question posted by Lloyd Graham on behalf of Lyn Schedlich>

Does anyone have details of reaction conditions suitable for labelling the
surface carboxylic acids of a protein with fluoresceinyl glycine amide in
the presence of EDAC and NHSS? The reaction is apparently done under
aqueous conditions, but the manufacturers supply the reagents without any
hints as to buffers, pH, concentrations, reaction time, etc., and
(incredibly) the literature references they cite don't supply this vital
information either.

Thanks,
Lyn.

************************************
DIRECT EMAIL REPLIES TO: lyns at med.su.oz.au

Lynette Schedlich
Kolling Institute of Medical Research
RNSH, St Leonards 2065, NSW, Australia
Phone No 61 2 99268486; Fax No 61 2 99268484
************************************


--Boundary (ID V9XX7MM22hBGr57OMsi93Q)
Content-type: TEXT/PLAIN


RFC-822-headers:
Received: from nexus.mc.duke.edu (nexus.mc.duke.edu)
 by mc.duke.edu (PMDF V5.0-5 #16741) id <01IHAT7ZUTWW0035MP at mc.duke.edu> for
 johns053%Phanes at ccmail.duke.edu; Fri, 04 Apr 1997 07:47:51 -0500 (EST)
Received: from net.bio.net (net.bio.net [204.31.212.2])
 by nexus.mc.duke.edu (8.7.5/8.7.3) with SMTP id HAA14509 for
 <johns053 at mc.duke.edu>; Fri, 04 Apr 1997 07:45:00 -0500 (EST)
Received: (from daemon at localhost) by net.bio.net (8.6.12/8.6.6)
 id EAA03772; Fri, 04 Apr 1997 04:29:28 -0800
Received: (from news at localhost) by net.bio.net (8.6.12/8.6.6) id EAA03764; Fri,
 04 Apr 1997 04:29:27 -0800
Date: Wed, 02 Apr 1997 07:04:37 +0000 (GMT)
From: graham at dbe.csiro.au (Lloyd Graham)
Subject: Labelling -COOH groups with fluorosceinyl-Gly-amide: help!
Sender: news at news.nsw.CSIRO.AU
To: methods at net.bio.net
Message-id: <graham-0204971811050001 at tastiger.dbe.csiro.au>
Content-transfer-encoding: 7BIT
X-Nntp-Posting-Host: tastiger.dbe.csiro.au

--Boundary (ID V9XX7MM22hBGr57OMsi93Q)--



More information about the Methods mailing list