Polyacrylamide gel electrophoresis query

Jacqui Whiteway jwhitewa at chat.carleton.ca
Mon Apr 7 09:19:29 EST 1997


M.R. Tock (BGYMRT at leeds.ac.uk) wrote:
> I was wondering if anyone could give me a concise answer to a 
> query I have regarding polyacrylamide gel electrophoresis 
> methodology.  

> The question is this ; when fixing gels with acetic acid & 
> methanol, what is happening ? (How does this fix 
> oligonucleotide bands ? By precipitation ? & are small oligos 
> likely to be leached out during this process ?).

> Many thanks in advance for your help.

> Mark. 

Hi,

Good question, I've wondered myself as most people in our lab don't fix
the gel, while some others do -- no difference in gel.  It does take less
time to dry after fixing though...  

Jacqui



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