biotin labelled oligonuleotide for southern blot

Shi-Jye Chu chusj at U.WASHINGTON.EDU
Mon Apr 7 14:36:48 EST 1997


Hi Everybody:

   Recently I like to do sourthern hybridization by using no-isotopic
oligonueleotide . But I like to use 5-end biotin labelled oligonuclide.
But I can't find the protocol for hybridization. Do any body had the
protocol ? Or this mean other non-iostopic label is better than biotin?

Welcome any suggestion !    Many thanks


S-J





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