immunofluorescence and haloarchaea

Andrew St. Jean andrew at bio01.bio.uottawa.ca
Tue Apr 8 10:46:22 EST 1997


Dear All,

I want to do immunofluorescence on a species of haloarchaeon, Haloferax
volcanii. I was wondering if anyone out there can help me with
permiablization protocols. Bear in mind that Haloferax has a
glycoprotein cell wall that does not contain peptidoglycan. Before it is
fixed, it will also be living in 2 M NaCl. If anyone has any input,
please email me. Thanks in advance.

Andrew St. Jean
University of Ottawa
andrew at bio08.bio.uottawa.ca



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