Looking for.....MethylMercury Hydroxide
BLIGHT at igmors.u-psud.fr
Tue Apr 8 03:26:07 EST 1997
I am trying out a method of SSCP mutation mapping that uses
direct ethidium bromide staining of ssDNA from PCR fragments
following denaturation and migration in a renaturing TBE gel
(Hongyo et al., 1993. Nucl.Acid.Res. 21(16): 3637-3642. The
trick to getting efficient denaturation of sufficient DNA to
stain requires the use of METHYLMERCURY HYDROXIDE.
Does anyone know of a supplier in FRANCE? I've checked
Sigma, Aldrich, Janssen, ICN & Kodak and can't find any.
Otherwise, if anyone has a better SSCP technique that is as
simple as staining the gels afterwards I'd be glad to hear
Thanks in advance.
PS I'd prefer replies by e-mail please.
Dr. Mark A. Blight
Institut de Génétique et Microbiologie,
CNRS URA 1354,
Université de Paris XI,
91405 Orsay cedex,
Tel: +33 1 69156699
Fax: +33 1 69157808
e-mail: blight at igmors.u-psud.fr
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