problem on PEG8000 in plasmid Extraction

nhwu at PUBLIC3.BTA.NET.CN nhwu at PUBLIC3.BTA.NET.CN
Wed Apr 9 10:22:54 EST 1997


Dear netter,

I am used to use the PEG8000/NaCl solution in large scale plasmid DNA 
purification.  It works well.  But recently I cannot see my DNA after adding PEG 
and centrifuging.  Another one in our lab has the preblem too: after he 
re-autoclave his PEG solution again he cannot get the DNA since then, although 
the PEG worked very well before. What happened to PEG?

Thanks for your any comment!

Xiao-yu

*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*
*								*
*	The Institute of Developmental Biology			*
*	Room 226,  PO Box 2707					*
*	Beijing 100080,  PR China				*
*	e-mail:nhwu at public3.bta.net.cn				*
*								*
*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*~*





More information about the Methods mailing list