Coating of in situ slides

M.Albalwi M.Albalwi at sheffield.ac.uk
Wed Apr 9 12:23:00 EST 1997


Stefan Nicolai wrote:
> 
> Hello,
> 
> I want to perform In situ Hybridisation on Rat Lung sections. There has
> been former experience in our lab on this subject, and I have most of the
> information, except on the pretreatment of slides. I know they were
> coated with 3-aminopropyltriethoxysilane (APES) and 2% gelatine.
> I know the gelatine coating part involves chromium potassium sulfate...
> 
> Can someone give me a protocol on exactly HOW to do this.
> 
> Please mail me at nicolai at uia.ua.ac.be
> 
> Thanks,
> ----------------------------------------------------------------------------
> Stefan Nicolai
> Dept. Biochemistry                                      Tel: +32 3 820.23.18
> University of Antwerp                                   Fax: +32 3 820.22.48
> Universiteitsplein 1
> B-2610 Wilrijk-Antwerpen                         Email: nicolai at uia.ua.ac.be
> Belgium                               WWW: http://www.uia.ua.ac.be/u/nicolai
> ----------------------------------------------------------------------------
Hello

You could try this reference, I am sure it will answer your question.

Journal of Clinical Pathology (1987), 40:page 1256-1260

Good luck

Balwi
Institute for cancer studies



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