Polyacrylamide gel electrophoresis query

Jeffrey Jackson jeffj at u.washington.edu
Wed Apr 9 19:39:19 EST 1997


On Mon, 7 Apr 1997, M.R. Tock wrote:

> I was wondering if anyone could give me a concise answer to a 
> query I have regarding polyacrylamide gel electrophoresis 
> methodology.  
> 
> The question is this ; when fixing gels with acetic acid & 
> methanol, what is happening ? (How does this fix 
> oligonucleotide bands ? By precipitation ? & are small oligos 
> likely to be leached out during this process ?).
> 

Fixing with acetic acid and methanol removes the SDS from the gel, which
in turn enhances coomassie staining.  

Jeff




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