IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Semi-quantitative RTPCR

Helen Haase h.haase at mailbox.uq.oz.au
Fri Apr 11 07:23:30 EST 1997

I would like to know more about validating my semiquantitative RTPCR 
system.  From the same RT sample I am doing two PCR reactions, one 
using primers for the message of interest and one for 
B2-microglobulin, which should act as a control.  The PCR reactions 
are done under the same conditions, at the same time.  I have 
established that the cycle number has been limited to ensure that 
amplification is still exponential.

Is it valid then to compare the intensity of staining of the ethidium 
bromide stained bands and express results relative to the B2M control?
Any references on principles involved would be appreciated

Thank you

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net