polyethylene glycol and dimethyl sulfoxide
genecutl at mendel.NOSPAM.berkeley.edu
Thu Apr 10 17:05:55 EST 1997
In article <97Apr9.133612edt.35721 at firewall.cedarcrest.edu>,
100 at cedarcrest.edu, College at cedarcrest.edu, Drive at cedarcrest.edu wrote:
< I received a protocol for bacterial transformation. The solution used
< for competence contained polyethylene glycol and dimethyl sulfoxide. I
< was wondering if anyone knows why these reagents are included in the
< protocol. What do they do to enhance competence and transformation?
< Thank you,
< Susan Walsh
< sjwalsh at cedarcrest.edu
DMSO helps to carry molecules across cell membranes. It's used
in things like nicotine patches. PEG is probably there to act
as a thickener. It increases the effective concentration of solutes.
Those are my guesses.
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