Help! Problem with Northern Blots!->Membrane swaps & Church hyb

Bradley Turner bsturner at MBCRR.HARVARD.EDU
Thu Apr 10 13:27:35 EST 1997


Hello Joy,

There has recently been discussion in this group and in the literature
about manufacturers changing the formulations of their nylon membranes
causing high background on blots.  Below I've included some of the emails
that contain the references to these discussions.  I recently spoke with
one of the suppliers about this and they did say that a change in their
nylon membrane formulation led to unacceptably high background hybridization
and that using "Church's hybridization buffer" as discussed below would
eliminate this.

I think that you can access the TiBS articles via the WEB at

http://www-Immb.ncifcrf.gov/~pnh/

(all kudos to Paul Hengen)

I hope this helps,
Brad Turner


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		    Bradley Turner
	        Beth Israel Deaconess
                    Medical Center

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			bsturner at mbcrr.harvard.edu
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Boston, MA 02215, USA		turner at sprcore.bidmc.harvard.edu
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JOY'S ORIGINAL QUESTION--------------------------------------------------



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