Help! Problem with Northern Blots!

pete wilson PETER.WILSON at BBSRC.AC.UK
Sat Apr 12 07:12:19 EST 1997


Reusing the electrophoresis buffer can cause this effect if the tank has
previously used with the target molecules of the probe you are using. The
contaminating molecules enter the gel from the top (-ve) so, if that is
what is happening, that end of the blot will be black.

Ian Dunn
Roslin Institute
EH25 9PS



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