protein expression systems
Lynn.Hughes at jcu.edu.au
Sun Apr 13 23:27:09 EST 1997
Hi all, I am just wanting some opinions on the different prokaryotic
expression systems available. I am attempting to express proteins
(100 kDa) for use as a vaccine and basis of an ELISA. For one of the
proteins I have I do not have the DNA for the entire open reading
frame, whilst the for the other I do. I have looked into the vectors
used for expressing with a His tag, and the GST gene fusion vectors.
I am wandering if anyone could tell me which of these would be more
appropriate for my needs, or what particulars of the vectors I need to
consider when making my choice.
Thanks for your help.
Dept of Microbiology and Immunology
James Cook University
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