Isolation of low copy plasmid

Andreas Vogel vogela at uni-muenster.de
Wed Apr 9 07:18:58 EST 1997


Hello all,
does anyone have experience to increase the yield of a miniprep from a low
copy plasmid (pET from Novagen, a pBR322 derivat) habouring E. coli
DH5alpha? Collecting multiple lysates on one spin column (Invotrogen`s SNAP)
yielded only poor quality DNA. Does Chloramphenicol-amplification works with
this vector?
Thanks,
Andreas




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