Isolation of low copy plasmid

Dr. Duncan Clark duncan at genesys.demon.co.uk
Tue Apr 15 03:50:46 EST 1997


In article <Pine.A41.3.96.970409141335.110978A-100000 at asterix.uni-
muenster.de>, Andreas Vogel <vogela at uni-muenster.de> writes
>Hello all,
>does anyone have experience to increase the yield of a miniprep from a low
>copy plasmid (pET from Novagen, a pBR322 derivat) habouring E. coli
>DH5alpha? Collecting multiple lysates on one spin column (Invotrogen`s SNAP)
>yielded only poor quality DNA. Does Chloramphenicol-amplification works with
>this vector?

Chloramphenicol will work or spectinomycin (50ug/ml final). It's not
really a low copy number plasmid. Now if you are talking pSC101 or
similar derivatives then that can be problem! We are all spoilt with pUC
copy levels.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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