His tagged proteins

Ian Goodfellow sas96igg at reading.ac.uk
Tue Apr 15 07:08:40 EST 1997


Hi all,

does anyone have any experience purifying His tagged proteins from yeast -
I have done this loads from other bugs but not from yeast and I am having
a hell of a time getting rid of other proteins which appear to co-purify.

I have tried dirfferent pH during purification - two types of resin
(Ni-agarose and Talon resin - Talon is cleaner) - I have also tried
immidazole in the binding buffer.

Any comments would be much appreciated

Ian



Ian Goodfellow
I.G.Goodfellow at reading.ac.uk




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