His tagged proteins
sas96igg at reading.ac.uk
Tue Apr 15 07:08:40 EST 1997
does anyone have any experience purifying His tagged proteins from yeast -
I have done this loads from other bugs but not from yeast and I am having
a hell of a time getting rid of other proteins which appear to co-purify.
I have tried dirfferent pH during purification - two types of resin
(Ni-agarose and Talon resin - Talon is cleaner) - I have also tried
immidazole in the binding buffer.
Any comments would be much appreciated
I.G.Goodfellow at reading.ac.uk
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