Northern problems

Tatjana Adamovic Tatjana.Adamovic at
Mon Apr 14 22:34:50 EST 1997

> We have been running Northerns for some years in our lab
> without any major problems using Hybond N, nylon membranes
> from Amersham.
> But since a couple of months our Northerns have failed. The 
> blots have high background which is irregularly spread
> on the membrane. We have tried several different hybridisation 
> and washing procedures without any success. 
> Now it was revealed that Amersham have changed the composition
> of the membrane due to some legal issues.
> Does anyone know of an other nylon membrane that is equal to
> the "old" Hybond N? Would be very grateful if you could give me
> the name of the manufacturer or the retailer.
> Thank you,
> Stanko Skrtic
> M.D., Ph.D.student
> stanko at

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