Low yield with Taq from GIBCO BRL.

Zhongtang Yu zyu at UNIXG.UBC.CA
Wed Apr 16 21:09:19 EST 1997


I have a problem with the Taq (from GIBCO BRL) to PCR applify
purified plasmid DNA (with the Qiaquick spun columns) below
10 to the 4th copies. Does anyone know why and how to increase
sensitivity?  I have not compare the Taq from GIBCO BRL with the
AmpliTaq from Perkin Elmer.  Which one is better in term of yields?
Any other suppliers provide good-yield DNA polymerase?


Zhongtang Yu
Microbiol., UBC.

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