Help - Poor reamplification of PCR products

Eliot Spindel spindele at
Wed Apr 16 17:49:42 EST 1997

In trying to amplify rare mRNAs, my first round PCR amplification works fine, 
but when I reamplify (taking .5 ul from a 50 ul reaction) I do not get any 
significant reamplification.  Any thougts?  Thanks.

Eliot Spindel

More information about the Methods mailing list