Help - Poor reamplification of PCR products

Eliot Spindel spindele at teleport.com
Wed Apr 16 17:49:42 EST 1997


In trying to amplify rare mRNAs, my first round PCR amplification works fine, 
but when I reamplify (taking .5 ul from a 50 ul reaction) I do not get any 
significant reamplification.  Any thougts?  Thanks.

Eliot Spindel



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