Q-B-replicase probing?

j.r.stephen mbi071 at abdn.ac.uk
Thu Apr 17 11:17:25 EST 1997


Hi Folks,

Anyone out there used Q-B-replicase probing for in situ detection of bacteria? I
am toying with the idea of using this method linked to fluorescent oligo probing
(after the Q-B replication step) to light-up different groups of bacteria in
mixed culture.

Any opinions/ advice? Anyone know of a kit? Anyone know of an email address for
Gen-Trak?

Thanks in advance,

John Stephen



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