Removing salt from DNA prep
rafael at howard.genetics.utah.edu
Fri Apr 18 14:51:44 EST 1997
>wise at bscr.uga.edu wrote:
>I'm doing a DNA prep from sediment from a stream and overall it is working
>well except that the obtained pellet contains large amounts of salt. I
>was wondering if anyone has any ideas about the best ways to remove salt
>from DNA in solution. I've tried washing the pellet with 70% ethanol at
>room temp. but it doesn't seem to help too much. thanks in advance!
Centricon o Microcon membranes is what you need. They are filters mounted
in tubes you can spin in centrifuges (centricon in a big centrifuge,
Microcon in a microfuge). If you choose the right pore size, your DNA
will be retained in the filter receptacle and your salt washed away.
Not relation with Amicon, etc.
Rafael Maldonado | 'No te creas todo
room 6160 Eccles Institute of Human Genetics | lo que leas en
Department of Human Genetics | internet.'
University of Utah |
Salt Lake City, Utah 84112. USA. | "Don't believe
Rafael.Maldonado at genetics.utah.edu | everything you read
Rafael at howard.genetics.utah.edu | in internet."
Tel: 801-581-4429 |
Fax: 801-585-3910 |
More information about the Methods