GC-rich region PCR

Greg Nattrass gnattras at waite.adelaide.edu.au
Thu Apr 17 07:08:06 EST 1997


I recently used eLONGase (Life Tech) to amplify a highly GC-rich 5' 
untranslated region. I had no success with any brand of Taq even when 
using various percentages of formamide or DMSO. eLONGase is also 
considerably cheaper than GC-Advantage from Clontech.

Best of luck
Greg Nattrass



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