Zero Background Cloning Kit from INVITROGEN

[Paul N Hengen]

Ramon Garcia Escudero (rgescudero at trasto.cbm.uam.es) wrote:

> Do anyone use the Zero Background Cloning Kit from INVITROGEN?
> 
> I am very interested in a fast and easy bacterial cloning system. 
> INVITROGEN have the new which which permits high levels of sensibility 
> and easy to use. CLONTECH have the new "Zero Background Cloning Ki" but 
> I don't know wheter it really works.

I think you mean the pZero vector from Invitrogen, not Clontech.
Have a look at the pros and cons of using this system in this article:

@article{Hengen1997Martibs,
author = "P. N. Hengen",
title = "Methods and reagents - Reducing background colonies
with positive selection vectors",
journal = "Trends in Biochemical Sciences",
volume = "22",
number = "3",
pages = "105-106",
month = "mar",
year = "1997"}

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