betaine in LA PCR

Dr. Duncan Clark duncan at
Sat Apr 19 04:15:57 EST 1997

In article <5j8n0i$98k17 at>, Paul N Hengen
<pnh at> writes
>BTW, they also tried Taq polymerase with 1-1.5 M betaine added and say it
>helped in yields of some longer PCR products. I'd be interested in seeing a
>comparison of 1-2 M betaine added to various buffers with and without K+
>(Woodford1995) and with and without DMSO (Filichkin1992) for Taq processivity
>and fidelity. 

One more reference, Biotechniques, Dec 1996, pp1102-1108. Note the last
two lines in the acknowlegements!

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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