betaine in LA PCR

Dr. Duncan Clark duncan at genesys.demon.co.uk
Sat Apr 19 04:15:57 EST 1997


In article <5j8n0i$98k17 at ncisun1-nf0.ncifcrf.gov>, Paul N Hengen
<pnh at ncifcrf.gov> writes
>BTW, they also tried Taq polymerase with 1-1.5 M betaine added and say it
>helped in yields of some longer PCR products. I'd be interested in seeing a
>comparison of 1-2 M betaine added to various buffers with and without K+
>(Woodford1995) and with and without DMSO (Filichkin1992) for Taq processivity
>and fidelity. 


One more reference, Biotechniques, Dec 1996, pp1102-1108. Note the last
two lines in the acknowlegements!

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.dnamp.com
http://www.genesys.demon.co.uk



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