C4 or C8 instead of C18 Reverse Phase HPLC in protein purification?

Lesley Woodhouse glyph at csolve.net
Sat Apr 19 19:02:24 EST 1997


Gel permeation chromatography can separate compounds based on moleclar
weight using HPLC.  This is a common way to separate proteins

Phil
glyph at csolve.net

newera at plaza.snu.ac.kr wrote in article <5j80aj$i24$2 at snunews.snu.ac.kr>...
> I am trying to purify a small protein(mw 6500) using 18C reverse phase
HPLC but I have failed until now because twe peaks are almost superimposed.
Various gradient programs do not work. 
> Is there any chance that C4 or C8 could separate them?
> 
> Thanks.
> 
> Lee, Ji Hyun
> 
> --
> email 	: newera at plaza.snu.ac.kr
> address :
>   Lee, Ji Hyun
>   Laboratory of Physical Pharmacy(Prof. Lee, Bong Jin)
> 
>   Seoul National University
>   College of Pharmacy		  
>   Shinlim-Dong, Kwanak-Gu
>   Seoul 151-742, Korea.
> 
> 



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