Background problems with Ni-NTA HRP conjugate

Sailesh Surapureddi Saisu at mcb.liu.se
Mon Apr 21 12:43:37 EST 1997


Hey! 

		Thanks for bringing this up.. I guess you have saved me from spending some
hard cash on this product. But before you junk this Ni-NTA conjugate.. I
would try to increase the dilution (say 1:2000) to decrease the background.
Pls do post your follow-up on this.

Btw, has anybody tried the Clontech's, monoclonal against 6XHIS to screen
for the expression of HIS tagged protiens.. If so I would like to hear your
comments on this one and only product.

Sailesh.



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