Background problems with Ni-NTA HRP conjugate

[Thomas R. Anderson]

In <335B945B.19E5 at bu.edu> reddypvr at BU.EDU (padma reddy) writes: 
>
>Hi. I am using the Ni_NTA HRP conjugate (from Quiagen) to screen cell 
>lines for the expression of his tagged proteins. I am getting a lot of
>background in both 3% milk-PBS-Tween and 3% BSA in PBS-Tween when used
>as a blocking agent. The Ni-NTA HRP conjugate is  diluted in PBS-Tween
>without milk/BSA as recommended. Any suggestions?
>Thanks
>Padma

One alternative would be to use an antibody against polyhistidine to
detect the tagged protein.  Several are available.  If you would like
information about hours, please let me know.

Tom Anderson
Berkeley Antibody Company  (BAbCO)
tel:  1-800-922-2226  (in the USA)
tel:  1-510-412-8930  (anywhere)
fax:  1-510-412-8940
email:  tanders at babco.com
www.babco.com