Labelling PCR fragment for Northerns

jjm30 jjm30 at dka.sm.ic.ac.uk.
Tue Apr 22 05:43:07 EST 1997


Hi all,   I have a 500 bp fragment generated by RT-PCR, whats the best
way (or easiest but good way) to radiolabel this for use as a probe in
Northern blotting to detect a moderate abundance message.  I've seen
the recent posts about labelling oligos but they seemed to be for
smaller (100 bp) fragments.  Is random priming the best way to go??



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