RFD: Plasmid re-ligation without ligase?
Sergey V. Orlov
Serge at usr1.iem.ras.spb.ru
Tue Apr 22 03:35:57 EST 1997
I have experience with transformation E. coli by linear plasmids.
It does work. The efficiency is lower (about 100 - 1000 times) than
in case of nondigested plasmids, but ligation in vivo exists.
By the way, it is impossible that Wolfgang's clones contain nondigested
plasmids, since their plasmids contain deletions.
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