RFD: Plasmid re-ligation without ligase?

Sergey V. Orlov Serge at usr1.iem.ras.spb.ru
Tue Apr 22 03:35:57 EST 1997


Dear Rafael,
I have experience with transformation E. coli by linear plasmids.
It does work. The efficiency is lower (about 100 - 1000 times) than
in case of nondigested plasmids, but ligation in vivo exists.
By the way, it is impossible that Wolfgang's clones contain nondigested
plasmids, since their plasmids contain deletions.

Sincerely, Serge.




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