PCR product cloning

Leyguarda leyguarda at ulysse.cea.fr
Tue Apr 22 07:44:53 EST 1997


In article <c-runyan-2104971438560001 at pc91.pediatrics.nwu.edu>, c-runyan at nwu.edu (Connie Runyan) says:
>
>I am trying to clone PCR products using blunt end ligation with the
>PCRScript kit from Stratagene.  This kit uses blue/white sceening for
>insert positives, but I have not had a lot of luck with this kit.  I am
>getting a lot of false positives in the few colonies that grow in the
>first place.
>
>I am interested in trying a different kit, and I would like to know if
>anyone has had success with any of the other kits out there.  I appreciate
>any advice.
>
>Thanks, Connie

We used the TA cloning kit from Invitrogen and it works very well. 
You can try it.



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