PCR product cloning

Baylor Molecular Genetics Lab Steven_Burghart at baylor.edu
Tue Apr 22 19:40:56 EST 1997


Hi Connie,
I am personally recommend you TA Invitrogen kit.
Very reliable and simple. So, I am voting for TA kit.
Yerlan
In article <c-runyan-2104971438560001 at pc91.pediatrics.nwu.edu>,
c-runyan at nwu.edu (Connie Runyan) wrote:

> I am trying to clone PCR products using blunt end ligation with the
> PCRScript kit from Stratagene.  This kit uses blue/white sceening for
> insert positives, but I have not had a lot of luck with this kit.  I am
> getting a lot of false positives in the few colonies that grow in the
> first place.
> 
> I am interested in trying a different kit, and I would like to know if
> anyone has had success with any of the other kits out there.  I appreciate
> any advice.
> 
> Thanks, Connie

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