Dr. Duncan Clark
duncan at genesys.demon.co.uk
Wed Apr 23 09:29:19 EST 1997
In article <c.rodemer-2304971116380001 at tiger.inet.dkfz-heidelberg.de>,
"C.Rodemer" <c.rodemer at dkfz-heidelberg.de> writes
>can I add Xgal and IPTG shortly after autoclaving LB media (and before pouring)
>Or are they heat inactivated then ?
I was always taught to add when media is no higher than 50C however I
know that Xgal can be used with on Thermus plates at 65-70C so maybe
it's not that critical.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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