Anyone using Peptide Nucleic Acids?
carl_hoeger at qm.salk.edu
Wed Apr 23 13:47:12 EST 1997
If you are working with PNA's I have a question for you. I am just starting
to be involved in a PNA project and as such I have to learn about how to do
the gel retardation assay that is carried out in this field. Specifically,
my questions are as follows:
1. What is the best electrophoretic system (in your hands) for doing these
gel retardation assays?
2. Do you have a protocol that you would be willing to share regarding the
complexing of PNA to DNA (for example, a PNA-C10 to a dG10) and the
3. Detection methods-do you have any other than radioactive ones?
Thanks in advance!
The Salk Institute
<hoeger at salk.edu>
More information about the Methods