Ammonium Sulphate ppt + His Tags

[Jared Head]

Furhter to the sensible sounding advice of using a simple Ammonium
Sulphate precipiation in conjunction with a nickel column His.Tag
purification, can anybody give me advice on how to set yourself up for
such a step.  What's the easiest and most systematic way of determining
what percentages of Ammonium Sulphate are needed for a particular protein; 
come to think of it, how exactly do you do the precipitation?  Is it best
before or after the nickel column?

Thanks in advance,

Jared

-- 
Jared Head     at the Department of Biochemistry, University of Bristol

"A computer lets you make more mistakes faster than any invention in human
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