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Ammonium Sulphate ppt + His Tags

Jared Head bijgh at zeus.bris.ac.uk
Thu Apr 24 09:24:36 EST 1997

Furhter to the sensible sounding advice of using a simple Ammonium
Sulphate precipiation in conjunction with a nickel column His.Tag
purification, can anybody give me advice on how to set yourself up for
such a step.  What's the easiest and most systematic way of determining
what percentages of Ammonium Sulphate are needed for a particular protein; 
come to think of it, how exactly do you do the precipitation?  Is it best
before or after the nickel column?

Thanks in advance,


Jared Head     at the Department of Biochemistry, University of Bristol

"A computer lets you make more mistakes faster than any invention in human
     history - with the possible exceptions of handguns and tequila." 
                                                   Mitch Ratliffe

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