PCR enzymes and Non-radioactive DNA sequencing

Bob Steinberg rsteinbe at etowah.uokhsc.edu
Thu Apr 24 16:26:46 EST 1997


michael Tilley wrote:
> 
> Does anyone have experience with UlTma the proofreading enzyme from PE?
> Would the fidelity offset the cost as compared to say Pfu?
> 
> Experiences with non-radioactive DNA sequencing, particularly Promega Silver
> stain kit. Although the Amersham system looks nice I do not have the ability
> for large transfers.
> 
> Regards
> MIKE
Beware-- the fidelity of ULTma is lower than for Taq



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