Ammonium Sulphate ppt + His Tags
ckwen at expert.cc.purdue.edu
Thu Apr 24 12:43:39 EST 1997
Jared Head (bijgh at zeus.bris.ac.uk) wrote:
: Furhter to the sensible sounding advice of using a simple Ammonium
: Sulphate precipiation in conjunction with a nickel column His.Tag
: purification, can anybody give me advice on how to set yourself up for
: such a step. What's the easiest and most systematic way of determining
: what percentages of Ammonium Sulphate are needed for a particular protein;
: come to think of it, how exactly do you do the precipitation? Is it best
: before or after the nickel column?
: Thanks in advance,
You have to titrate with different amount of Ammonium sulphate to determine.
Probably start from 10%, 20% 30%..... and test which % gives you the best
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