YAC transformation efficiency problem

Martha L. Reed mlr8 at cornell.edu
Fri Apr 25 10:21:59 EST 1997


In order to protect large DNA insert in YAC library production, we use
polyamine in the transformation (Larin, et al. YAC Protocols 1996).  But
this reduces the efficiency of transformation by more than ten fold when
tested on vector alone.  Has anyone managed to find a way out of this
dilemma?



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