Primer extension analysis

Paul W. Diaz, Ph.D. ghabets at onyx-pharm.com
Fri Apr 25 07:10:39 EST 1997


Most likely your product is just "garbage" to use your term.  
If after your primer extention reaction you are denaturing 
your sample properly a 300 bp ssRNA or ssDNA will run 
cleanly on a 29:1, 7M urea gel.  Formamide is not required.

pendragon



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